![]() ![]() The negative control is expected to result in no amplification of the target regions. Additionally, to prevent the reporting of false positives, negative controls are run during each experiment to ensure contamination is identified if it does occur. Care must be taken to avoid contamination of reagents with genetic material from samples, kit controls, the environment, or amplicons from previous reactions. This same sensitivity also makes PCR assays very sensitive to contamination and can easily deliver false positive results unless an appropriate negative control is used in the assay. Negative Controls – Preventing False Positives These aid in the interpretation of results by identifying contamination during processing, inhibition of the reverse transcription and amplification reactions, or even if the pre-PCR step of extraction was successful or not When used for pathogen detection, RT-PCR assays require the use of appropriate controls. This sensitivity makes the assay ideal for identifying the presence of this specific coronavirus in a sample. PCR is extremely sensitive and only trace amounts of the template DNA or RNA are necessary for identification. RT-PCR assays reverse transcribe the viral RNA into DNA for amplification and subsequent identification of target regions. Real-time reverse transcription polymerase chain reaction (RT-PCR) assays are the tool of choice for determining if someone has an active viral shedding of SARS-CoV-2. PKamp Monkeypox Real-time PCR RUO Kit V1Īccuracy of SARS-CoV-2 testing is critical when determining if someone is infected and needs to be quarantined and/or treated for a coronavirus infection.Labchip GXII Touch Protein Characterization System.Solutions for Labs Performing miRNA Services.explorer Integrated Workstations for SARS-CoV-2 Testing.JANUS G3 Workstations for SARS-CoV-2 Testing.PKamp VariantDetect SARS-CoV-2 RT-PCR Assay.PerkinElmer GSP/DELFIA Anti-SARS-CoV-2 IgG Kit CE-IVD.Respiratory SARS-CoV-2 RT-PCR Panel CE-IVD.SARS-CoV-2 Plus RT-qPCR Reagent kit – CE-IVD.PerkinElmer COVID-19 Antigen Test – CE-IVD.PKamp Respiratory SARS-CoV-2 RT-PCR Panel 1 EUA. ![]()
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